Sugar industry is the backbone of rural economy of India. Sugar mill industry releases huge amount of effluent. In this study we have tried to evaluate the impact of sugar mill effluent on physical parameters and biochemical studies of black gram. Shoot length, root length, plant height, number of leaves, leaf length, carbohydrates, proteins, amino acids, chlorophyll, moisture, ash, iron and reducing sugar were also calculated. Results showed that the physical parameter and biochemical studies of black gram decreased in sugar mill effluent plant compared with normal water plant. Thus the present study can be concluded that the effluent cannot be utilized for irrigation.