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VOL. 10, ISSUE 6 (2025)
Efficient micropropagation of the medicinal orchid Pholidota imbricata Hook. f. from pseudobulb and leaf explants in Bangladesh
Authors
Awishik Tripura, Somaya Emrog Nayma, Md. Shahin Islam, Tapash Kumar Bhowmik
Abstract
Pholidota imbricata Hook. f. is a medicinally
significant epiphytic orchid native to Bangladesh, currently facing population
decline due to habitat destruction and over collection. This study establishes
a reliable and efficient protocol for the mass propagation using pseudobulb and
leaf explants derived from in vitro seedlings. The seeds were germinate in
vitro on agar solidified four basal media viz. KC, MS, MVW and PM
and produce a substantial number of plantlets, which were subsequently used for
micropropagation studies. Murashige and Skoog (MS) medium supplemented with
various concentrations of 6-Benzylaminopurine (BAP), Kinetin (Kn),
α-Naphthaleneacetic acid (NAA), Indole-3-acetic acid (IAA) and Picloram were
evaluated for shoot induction and proliferation. Direct organogenesis was
observed from pseudobulb segments, with the highest frequency of multiple shoot
buds (MSBs) formation (90.67% ± 1.96) recorded on MS medium supplemented with
2.0 mg/l BAP and 1.0 mg/l NAA. Conversely, leaf segments underwent somatic
embryogenesis, producing protocorm-like bodies (PLBs) with a maximum induction
rate of 74.67% ± 2.24 on the same hormonal combination. For shoot elongation,
agar solidified MS medium fortified with 1.5 mg/l BAP and 0.9 mg/l NAA proved
superior to liquid media, yielding the highest shoot length increase (2.72 ±
0.03 cm). Rooting was most effective on half strength MS medium supplemented
with 1.0 mg/l IAA and 1.0 mg/l IBA, resulting in a mean root number of 4.33 ±
0.33 and root length of 3.85 ± 0.03 cm. Following acclimatization, 75% of the
plantlets survived and were successfully established in the greenhouse. This
protocol offers a viable strategy for the commercial propagation and ex situ
conservation of this near threatened orchid species.
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Pages:31-36
How to cite this article:
Awishik Tripura, Somaya Emrog Nayma, Md. Shahin Islam, Tapash Kumar Bhowmik "Efficient micropropagation of the medicinal orchid <i>Pholidota imbricata</i> Hook. f. from pseudobulb and leaf explants in Bangladesh". International Journal of Academic Research and Development, Vol 10, Issue 6, 2025, Pages 31-36
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