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VOL. 8, ISSUE 5 (2023)
Prevalence and antibiogram of pseudomonas species isolated from selected African catfish (Clarias Gariepinus) ponds in Owo Metropolis
Authors
Adeluwoye Ajayi Olayemi Amos, Aladejimokun Adeniran Olusanya, Omoniyi Oluwasegun Victor
Abstract
One of the most common sources of protein globally is catfish which are
artificially reared in Nigeria. Pseudomonas species are opportunistic in
nature, pathogenic Gram negative bacteria and may build multidrug resistant which
can cause a serious threat to public health when present in environmental
samples. This research was meant to determine the antibiogram of Pseudomonas species
and its prevalence isolated from catfish ponds in Owo metropolis.
The pond’s water samples were aseptically collected from three ponds at
three different locations in Owo. Centrimide Agar was used for the Isolation of
Pseudomonas species, as well as Nutrient Agar, MacConkey Agar, and Eosin
Methylene Blue Agar were used for their morphological and cultural
characteristics, and the isolates were therefore characterized. Kirby Bauer
disc diffusion method was adopted to carry out Antibiogram of the isolates. The
isolates indicated different morphological and cultural characteristics. Prevalence
of the Pseudomonas species reported were: 43%, 33% and 23% accordingly.
The isolates were susceptible to the carbapenem (imipenem, 100%), monobactam
(aztreonam, 96%), nitrofuran (nitrofurantoin, 93%), aminoglycosides
(gentamicin, 90%), cephems (cefotaxime, 90%, ceftazidime, 93%, cefuroxime,
86%), fluoroquinolones (ciprofloxacin, 70% and ofloxacin, 63%) respectively. meanwhile,
the susceptibility of the isolates to beta-lactam combination appeared to be
low (augmentin, 30%), while carbapenem (Imipenem) was the most highly active
antibiotic, followed by the monobactam (aztreonam). This research revealed that
Pseudomonas species were common in catfish ponds and were differently
resistant to some of the tested antibiotics, thus poses threat to public health
which raises the need for serious monitoring of catfish ponds.
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Pages:19-24
How to cite this article:
Adeluwoye Ajayi Olayemi Amos, Aladejimokun Adeniran Olusanya, Omoniyi Oluwasegun Victor "Prevalence and antibiogram of pseudomonas species isolated from selected African catfish (Clarias Gariepinus) ponds in Owo Metropolis". International Journal of Academic Research and Development, Vol 8, Issue 5, 2023, Pages 19-24
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