The study of sucrose extraction from the optimal mix feedstocks; Corn stover, sugarcane waste and Bambara groundnut chaff by enzymatic hydrolysis was carried out. The proximate and compositional analysis of the feedstocks were done to determine the viability of each feedstock for sucrose production. Hydrogen peroxide solution (2.5%) was used for delignification pre-treatment of the feedstocks and optimal mixing ratio of the feedstocks was determined for maximum sucrose yield and recorded as (1:3:1) of corn stover, sugarcane waste and Bambara groundnut chaff. Effect of process parameters such as temperature, time, enzyme concentration, and pH were investigated and optimized using central composite design of response surface methodology. The kinetics of the enzymatic hydrolysis of the mixed feedstock was studied using michaelis-menten models. The results obtained showed that all the feedstock contained both cellulose, hemicellulose and lignin in quantities recommended for Sucrose production with cellulose being dominant. It was observed that the yields of sucrose increased as the parameters increased up to certain levels before decreasing. The optimal conditions obtained from the enzymatic hydrolyzed mixed feedstock are hydrolysis temperature of 35oC, enzyme concentration of 8g/50mol, contact time of 5days and pH of 5.5 with sucrose yield of 71.41%. The kinetic model of the enzymatic hydrolysis of the mixed feedstock agreed with the Michaelis-Menten model with activation energy of 11.267KJ/mol. This study has shown that corn stover, sugarcane waste and Bambara groundnut chaff mixed in the ratio of (1:3:1) can serve as an alternative raw material for the Sucrose production industries in Nigeria and the kinetic data can be used in the design of process equipment for large scale production.