Introduction: The HIV and AIDS pandemic presents to the world one of the most challenging and serious public health issues. The burden of HIV and AIDS are felt at all levels of the human society, including individual, family, community, workplaces and nations manifesting in the forms of poverty, increased unemployment rates. Tackling the problem of workplace HIV and AIDS-based stigmatization, bias and refusal; and diminishing the effects of HIV and AIDS on labour and productivity depend tremendously on executing successful HIV and AIDS progressive policies and programmes for the workplace organization. Objective: This study was carried out to assess the development and implementation of the workplace HIV and AIDS policy and programmes in Calabar Metropolis of Cross River State, Nigeria. Methods: The study was a cross-sectional descriptive survey of 112 private and public organizational managers of formal employers’ organizations. A semi-structured questionnaire was used to collect data from the respondents. The collated data was analysed using the Statistical Packaged for the Social Sciences (SPSS) software (version 16), expressed as frequencies and percentages, and presented in tables and charts. Results: The major findings revealed that 84.7% of the respondents were from the public sector. Only 10.2% of the organizations had workplace HIV and AIDS policy documents. Majority of the organizations (86.7%) were not implementing any workplace HIV and AIDS programme. Majority of the respondents (96.4%) stated that developing and implementing organizational workplace HIV policy and programme will cost money, 78.6% of the respondents identified time, 88.1% pointed out personnel, 47.6% of the respondents identified output reduction, disruption of the organization’s activities was identified by 75% of the respondents, and 82.1% identified materials as some of the costs in developing and implementing workplace policy and programmes on HIV/AIDS. Conclusions: only a few organizations have developed and are implementing workplace place HIV/AIDS policies and programmes. Money, materials, disruption of organization’s activities, and output reduction, were identified as barriers to the domestication of National workplace HIV/AIDS policy. The Nigerian government should provide employers’ organizations with the necessary support needed to domesticate the HIV/AIDS workplace policy.