The purpose of the study was to know the Relationship of selected physiological variables with volleyball playing ability among intercollegiate volleyball men players. Methodology- In order to achieve the purpose of the study forty (40) inter-collegiate volleyball men players were selected as the subjects. During inter collegiate tournament of Visvesvaraya technological University from the data pertaining to the four selected physiological variables such as vital capacity was assessed with the help of dry spirometer, pulse rate, systolic blood pressure and diastolic blood pressure was assessed with the help of Omron automatic blood pressure monitor MX3. Volley ball playing ability was assessed through subjective rating, by three experts, during the intercollegiate tournament and the average was taken as criterion score. Statistical Analysis-the collected data was tested with coefficient of correlation statistical technique to test the Relationship of selected physiological variables with volleyball playing ability among intercollegiate volleyball men players. Results-The statistical analysis shows coefficient of correlation in the Relationship of selected physiological variables with volleyball playing ability among intercollegiate volleyball men players. Conclusion-The analysis of the study revealed that there was a significant Relationship of selected physiological variables such as diastolic blood pressure and there was no significant relationship between selected physiological variables such as vital capacity, pulse rate, and systolic blood pressure with volleyball playing ability among intercollegiate volleyball men players.