Cycle sport is competitive physical activity using bicycles. There are several categories of bicycle racing. in bicycle short races produce lot of fatigue in muscles such as lactic acid and muscle stiffness. To find the exact effect the study conducted to determine the effect of commercial energy drinks on the depletion of blood lactate of interuniversity level cyclists. The design of the study was pre – post control group. This experimental study was carried out on 40 fit male cyclists who participated in all India inter university championship and cyclists divided into four equal groups. they were given a standardize energy drink such red bull group, gatorade group, rio group and control group, before the exercise researcher check the pre-examination on lactic acid variable. After performing they schedule cyclists was post examined on the lactic acid variable by using lactate pro portable blood lactate analyzer. For the results of the investigation analysis of covariance was used to find out mean differences between pre and post- test with the help of statistical package for the social sciences (spss) 16.0. the findings on pre-test mean values on blood lactate acid of red bull group, gatorade group, rio group and control group was 2.950, 2.860, 3.220 and 3.310 respective. the obtained ‘f’ ratio of 1.652 for pre-test scores is less than the table value of 2.87 for df 3 and 36 required for significance at.05 level of confidence on blood lactate acid and the post-test mean values on blood lactate acid of red bull group, gatorade group, rio group and control group was 4.560, 5.250, 4.650 and 4.707 respectively. The obtained “f” ratio of 1.274 for post-test scores is less than the table value of 2.87 for df 3 and 36 required for significance at.05 level of confidence on blood lactate acid. The result of the study shows that there was an insignificant effect of drinking gatorade group, rio group, red bull group and control groups on blood lactate acid. After that the F-ratio was found to be significant, the Scheffe’s post-hoc test for differences between the paired adjusted final means is use in this investigation. The mean difference.690 between Group- gatorade and Group-rio was found significant in favour of Group- gatorade. However, the differences for the remaining paired means were not significant.