The specific objective of the study was to identify determinant factors that affect sesame market supply in Kafta Humera Werda, Tigray Regional state, Ethiopia. In order to acquire the relevant data for this study both primary and secondary data were used. Cross sectional data was collected from 388 sample respondents of the Kafta Humera Werda Tigray regional state using multistage sampling technique. Data were analyzed using multiple liner regressions model to arrive at conclusion. The result of the multiple regression model indicates that sesame market supply was significantly and positively affected by total land cultivated, quantity of sesame produced, access to extension service, yield per hector, formal educational level of household heads, family size aged 18 to 69 years old, oxen ownership, sesame production experience,market information, and access to credit service at 1 and 5 percent significance level respectively. On the other hand, distance to market significantly and negatively affected sesame supply to market at 5 percent significance level. Strategies that are targeted at providing farmers with adequate credit and other extension services, improving marketing infrastructures, offering farmers a fair price, building experience of farmers, establishing institutions that disseminate reliable market information improving farmer’s linkage with cooperatives, while designing any marketing program are recommended to increase sesame market participation and marketed surplus in the study area.Policy makers should focus on these socio-economic and institutional factors influencing the farmer’s market choice. This in turn may reduce food security problem and enhance income of the farmers. Since, land under sesame is a proxy measure of quantity of sesame produced the area used for sesame production is difficult to increase due to its limited resource. Therefore, it is better to increase the productivity of sesame. Adequate supply of improved varieties, controlling of disease and pests and use of optimum input should be applicable to increase the productivity of sesame and its supply to the market. Thus, the government and/or private sector players should train farmers on these areas. Training on production and post-harvest handling techniques could address this challenge. Economical support should be given to farmers through formal credit agencies. Strong extension intervention is vital to assist farmers in producing high quality sesame and increase production through consistent follow up, and keeping of farm records.In suggesting upgrading strategies to improve chain competitiveness and efficiency, productivity and quality are becoming more important for sesame farmers to compete in an increasingly competitive market. Therefore, these factors must be promoted by developing farmers’ awareness about marketing and post-harvest handling, developing storage infrastructure and coordinating fragmented producers in to farmer cooperatives.