Thespesia populnea L, also known as ‘Portia tree’ is in the Family of Malvaceae. Here we try to evaluate the antimicrobial activity of these extracts against Staphylococcus aureus and Streptococcus pyogenes which are opportunistic pathogens in HIV/AIDS patients Dried powdered flower extracts derived by using Ethyl acetate and acetone. The extracts were subjected to Gas Chromatography and Mass Spectrophotoscopy (GCMS) analysis. A qualitative phytochemical analysis was performed for the detection of alkaloids, phenolic flavonoids, carbohydrates, aminoacids, proteins, anthroquinones, saponins, tannins, phylobotannins, glycosides, cardiac glycosides, anthocyanides, terpenoids, and steroids. These extracts were tested against Staphylococcus aureus and Streptococcus pyogenes and antimicrobial activity was estimated by turbidometry and well diffusion method. When a software application was used for docking of antimicrobial compounds, it was observed that the extracts showed antimicrobial activity against the microorganisms of our study. This result suggests that T. populnea L flower contains phytochemicals with medicinal properties.