Background: World Health Organization reported that worm infestation is one of the common health problems worldwide especially among children. WHO estimated that about 1400 million people worldwide are infected with at least one type of Intestinal worm. Hook worm, Pin worm and, Tape worm commonly acquired orally or parentally or both routs. Objectives: 1. To assess the knowledge regarding prevention of worm infestations among mothers of under five children. 2. To find out the association between the level of knowledge regarding prevention of worm infestation with selected socio demographic variables. Materials and Methods: The Non-experimental descriptive design with non probability Convenience sampling technique was adapted to select 60 mothers of under five children in Venkatachalam at Nellore District. Results: The result shows that among 60 samples 60(98.4%) of mothers have inadequate knowledge and 1(1.6%) of mothers have adequate knowledge regarding prevention of worm infestation.