The present study was carried out to evaluate the antibacterial and antihelminthic property of Cassia senna root bark. Root bark extract was prepared by using Soxhlet apparatus and ethanol was used as solvent. The phytochemical screening of the extract was performed which revealed the presence of Phenols, Flavanoids, Steroids, Glycoside, Saponins and Alkaloids. The extract was subjected for antibacterial screening against a gram negative (Escherichia coli) and a gram positive (Staphylococcus aureus) bacterial strains using cup plate method. The results obtained were compared with Streptomycin which was used as reference drug. The extract was also screened for antihelminthic activity by using earthworms.