Many disease initiated by the Bacteria in mammals, therefore they may effect on immune system, that’s why it is important to search for new types of antibacterial agents. The main purpose of this study is to test antibacterial activity of skin secretion derived from two species of anurans endemic found in India (Sirohi), Bufo melanostictus and Rana cyanophylictitus. The toads and frogs were induced to secret skin secretion without using any stimulant. The collected skin secretion was filter and sterilized and finally subjected to antibacterial assay. Results showed that skin secretion of some toads and frogs has antibacterial activity against the Clarithromycin. Some chemical compounds with high potential activity were detected in skin secretion include fatty acids, steroids and amines. Therefore, the toad and frog skin secretion has the potential to be developed as a source of antibacterial agents.