Many disease initiated by the Fungal in mammals, therefore they may effect on immune system, that’s why it is important to search for new types of antifungal agents. The main purpose of this study is to test antifungal activity of skin secretion derived from two species of anurans endemic found in India (sirohi), Bufo melanostictus and Ran acyanophylictitus. The toads and frogs were induced to secret skin secretion without using any stimulant. The collected skin secretion was filter and sterilized and finally subjected to antifungal assay. Results showed that skin secretion of some toads and frogs has antifungal activity against the Penicillium. Some chemical compounds with high potential activity were detected in skin secretion include fatty acids, steroids and amines. Therefore, the toad and frog skin secretion has the potential to be developed as a source of antifungal agents.