To screen hexane, methanol and water extracts of eight types of ethno medicines and four types of oils used in traditional medicines in Mandla district (Madhya Pradesh, India) against three keratinophilic bacterial species viz., Bacillus subtilis, Escherichia coli and Enterococcus faecalis. Disc diffusion method was used to determine the inhibition zone of different extracts. Hexane, methanol and oils showed significant zone of inhibition against Gram-positive bacteria Bacillus subtilis and Enterococcus faecalis then Gram-negative bacterium Escherichia coli. The hexane extracts of the studied plants were more potent than the methanol extracts. Oils showed significant activity than other medicinal preparations. The most susceptible bacterium was B. subtilis followed by E.faecalis and E. coli was least inhibited. Crude water, hexane and methanol extract of some preparations were not showing inhibitory activity against bacterial species. Based on the literature, this is the first study which has conducted to inhibit growth of keratinophilic bacteria. Further research also needs to purify and characterize the secondary metabolites from the selected oils for the valuable source of novel substances for future drug discovery.