Disinfection and sterilization in hospitals is a significant concern for both the medical and the general community. Disinfection is a process that reduced or completely eliminates all pathogenic micro-organisms. Very few studies have been done in India quoting the importance of CSSD in the prevention of infections and control. This study was under taken to assess knowledge regarding disinfection of hospital equipments among health personnel in a tertiary hospital of Jammu, J&K. The sample consisted of 100 health personnels. Purposive sampling technique was used to select the sample. Socio-demographic profile and Self structured questionnaire was used to collect data from the subjects. The results showed that that maximum subjects were having average knowledge (47%) followed by 26% were having very poor knowledge, 23% and 4% were having poor and good knowledge respectively.