Sensitive, spectrophotometric, simultaneous equation method (Method A) and Q-analysis method (Method B) have been developed for the simultaneous estimation of Darunavir ethanolate (DAR) and Ritonavir (RIT) in combined dosage form. The estimation by method A was carried out at the wavelength of 267.50 nm and 241.00 nm for DAR and RIT respectively. Method B involved the formation of absorbance equation at 244 nm (isoabsorptive point) and at 267.50 nm the maximum absorption of DAR. The linearity was found to be 8-35 μg/ml for DAR and 5-70 μg/ml for RIT by both methods. The recovery study of method a in tablet were found to be 102.99±1.22% and 101.79±1.46% for DAR and RIT respectively. The recovery result of DAR and RIT were found to be 101.93±0.79% and 99.04±0.98 for method B respectively. Both the methods were validated as per ICH guidelines.