Achievement motivation plays a vital role for success in academic settings. The present study was carried out with the objective to investigate the gender and medium of instruction related differences. The study was carried on 500 secondary school students of various schools from Hyderabad region, 238 boys and 262 girls selected by random sampling method. As per research plan all students were selected on the basis of gender (males and females) and medium of instruction using Achievement Motivation Scale. Percentage analysis and t- Test were used for deriving the results. Significant differences were found in the dimensions of achievement motivation on the basis of gender and medium of instructions among boys and girls students.