This article was aimed to investigate the personality of Urban and Rural Handball Players representing All India Inter University and its relationship with sport performance. The method of the study is descriptive correlational. The data was collected using questionnaires and through field study procedure. The population of the study consisted of 324 urban and 176 rural Handball Players representing All India Inter University. Personality was assessed using the Personality questionnaire constructed by Eysenck’s Maudsley Personality Inventory (MPI) questionnaire by S. S. Jalota and S. D. Kapoor (English version) (1959). T-test was used to compare the groups at 0.05 levels of significance. On the basis of result of the present study, it was concluded that urban handball players are more extrovert than rural handball players and rural handball players are more neurotic than urban handball players.