The purpose of the present study was to investigate the effect of Fartlek training on, speed and endurance among physical education students of Annamalai University. To achieve the main purpose of the study, 40 male students were selected from Annamalai University which serves as subjects and their age group is between 18 to 25 years. The selected subjects were divided into two groups, experimental group and control group. 20 male student were selected from each group which serves as subjects. The experimental group goes through special Fartlek training practices, for six weeks which was considered adequate to show changes if any. The control group was not given any special training programmer apart from their regular activities. The physical variables which were examined during the study were speed and endurance. All the data which was collected during training programmer were examined by analyzing "t" test to find out whether any significance difference is found between the means of pre and post-test score of both groups within the period of six weeks of Fartlek training programmer. The level of confidence was fixed at 0.05.