Nasocomial infection causing significant mortality and morbidity among neonatal population especially high incidence among low birth weight and pre term babies. The present cross sectional descriptive study was conducted for identifying knowledge and practices regarding the care of neonates among staff nurses. 30 staff nurses were selected by using non probability convenience sampling technique. Strctured questionnaire and checklist was used for assessing knowledge and practice of staff nurses. Data was analyzed by using descriptive and inferential statistics. The results revealed that majority of 13(43.3%) had adequate knowledge and 29(96.67%) had satisfactory practices in providing neonatal care. The study concluded that there is a need to conduct CNE programmes to update nurse’s knowledge regarding aseptic technique and delivering effective services to neonates.