Psoralea corylifolia L. belongs to Fabaceae family is a traditional medicinal plant in India, commonly known as “Bachi”. It is used as a diuretic, diaphoretic, laxative and stimulant. The powdered seeds were applied externally to cure skin problems. It is valued as Chinese herbal medicine as a tonic remedy and is used to improve vitiligo. In the present study, the methonalic extract of the leaf callus of Psoralea corylifolia L was investigated for its phytochemical and HPLC analysis. Finding of this study revealed that the methanolic extract of Psoralea corylifolia L. leaf callus shows presence of flavanoids, phenolic coumpound and antioxidant activities calculated TFC, TPC and DPPH activity is 100 µg^-mlQE/100g, 670 µg^-mlGA/100g and 77.31% respectively and HPLC analysis confirms the presence of daidzein (111µg^-ml) and genistein (0.5µg^-ml) in methanolic extract of P.corylifolia leaf callus.