The study aims are to assess the knowledge regarding care of patient with dyspnea among staff nurses and nursing students and to identify the relationship between the knowledge level and socio demographic variables of staff nurses and student nurses. The quantitative research approach and cross sectional descriptive design was adopted for the study. The study was conducted in Narayana medical college hospital.15 staff nurses and 15 nursing students were selected by using Non Probability Convenience Sampling Technique. Semi structured Questionnaire method was used for collecting the information from samples. Data analysis done by using descriptive and inferential statistics. The results revealed that 1 (6.7%) staff nurse have inadequate knowledge, 9(60%) staff nurses have moderately adequate and only 5 (33.3%) staff nurses have adequate knowledge regarding care of patient with dyspnoea. 4(26.7%) have inadequate knowledge, 9 (60) students have moderately adequate and only 2 (13.3%) have adequate knowledge regarding care of patient with dyspnoea.the study concluded that majority of staff nurses and nursing students has moderately adequate knowledge and age, educational qualification and attending to in-service education of staff nurses has shown significant association with level of knowledge. Student’s socio demographic variables have shown non-significant association with the level of knowledge.